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The inhalation exposure of male F344 rats to respirable silica (RSC) was conducted by inhalation of a range of silica-containing particles (19 nm-2.5 µm), in conditions similar to those used in inhalation studies that have been conducted previously in our laboratory ( e.g. Holst et al., 1985 ; Holst, 1987 ; Otto et al., 1989 ; Otto, 1991 ). For example, the distribution of silica throughout the alveolar space is considered to be similar to that which results from exposure to respirable aerosols generated in shaker-type nebulizers. Inhalation exposures were conducted inhalted-flow inhalation exposure chambers. The study design typically involved 4 rats/exposure group for 4 days performed in 2-week exposure protocols. Procedures involving the use of experimental animals have been reviewed and approved by the National Institute of Health’s Animal Welfare Assurance Agency’s Office and are in accordance with the Guidelines for Care and Use of Laboratory Animals ( Institutional Animals Protocol, 1996 ). At the end of the exposure protocol, the animals were sacrificed and the lungs were examined by light and electron microscopy to identify the presence of silica particles. The particulate samples collected from animal lungs were either dry-wet-moist (DWM) or wet-dry-moist (WDM) preparations, and analysed by energy- dispersive X-ray fluorescence spectrometry (EDXRF) and chemical analysis. In addition, aliquots of the distilled lung washings were analyzed to determine the aflatulent content of quartz particles. In the dry-moist preparation, chemical analysis was also used to detect secondary silica compounds such as iron oxide or manganese oxides ( Joost et al., 1989 ).

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. that is, the finely divided particles of silica that are produced by action of heat upon crystalline. though for the most part these silica powders are so fine they are practically dust.. After attempting a few solutions I came up with using silicone oil as the wetting agent. By means of a wide-angle X-ray diffractometer and. If the silicone oil is left in contact with these prewarmed silica powders for a few days, the liquid mixture will. substances are silica and.
merck silica sample mg/ml
. (A) Silica (SiO2); (B) Silicic Acid (H4SiO4);. The peak assignments were made using the standard databases of diffraction pattern data provided. .
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